Journal: Advanced Science

Article Title: Multimodal Optical Imaging and Modulation with Simultaneous Electrophysiology Through Smart Dura in Non‐Human Primates

doi: 10.1002/advs.202514419

Figure Lengend Snippet: Multiphoton imaging through the Smart Dura. a) Schematics of bench‐side and in vivo multiphoton imaging of pollen and cortical vasculature. The Smart Dura electrodes are facing downward toward the fluorescent sample, which is the same condition as when recording neuronal signals from the brain. For in vivo imaging, a custom 3D‐printed imaging well with an 18 mm diameter glass bottom was secured onto the Smart Dura to immerse the objective lens in DI water and flatten the target area. The image at a depth of 0 µm is the plane focused on the metal traces. b) Two‐photon imaging of pollen grains placed under the Smart Dura. 3D reconstruction of the two‐photon images (left panel) and images at depths of 0 (middle panel) and 219 µm (right panel). The orange and blue arrows in the middle panel indicate metal traces with widths of 10 and 50 µm, respectively. c) In vivo vascular images at depths of 0, 100, and 200 µm in V1 under the Smart Dura using two‐photon microscopy. The orange and blue arrows in the left panel point out metal traces with widths of 10 and 50 µm, respectively. d) Normalized intensity profiles at depths of 0, 100, and 200 µm (from top to bottom) along the white dashed line shown in the image of 0 µm. The areas highlighted in orange and blue represent the regions where the metal traces of 10 and 50 µm width pass through, respectively. e) Three‐photon images of the vasculature under the Smart Dura and normalized intensity profiles along the white dashed line shown in the image of 0 µm. The orange shaded area is the region where the 10 µm‐wide metal trace passes through. The images were taken from the area highlighted in the red dashed box in Figure . f) Capillaries in a three‐photon image taken through the Smart Dura at a depth of 300 µm and intensity profile along the red dashed line. All ticks in the 3D image and scale bars in 2D images: 100 µm.

Article Snippet: The vasculature pattern was also acquired with the same imaging setup under 530 nm illumination (M530L4; Thorlabs) for later alignment of Smart Dura electrode contact positions.

Techniques: Imaging, In Vivo, In Vivo Imaging, Microscopy